Barry J. Saville B.Sc. (Guelph), M.Sc. (Toronto), Ph.D. (Toronto)
Associate Professor

I have been interested in agriculture since my early childhood in Lindsay Ontario where I worked primarily in horticulture. As an undergraduate student I became interested in the analysis of plants and their interactions with microbes. During my graduate student education my focus was to learn more about molecular biology and how it could be used to study biological interactions. This led to an interest in fungal molecular biology. During my postdoctoral research I began to get back to my roots investigating bacteria and fungi that interact with plants in beneficial and detrimental ways. This lead to a strong interest in biotechnology and I left academia to work with Applied Biosystems for two years before joining the faculty at the University of Toronto Mississauga. While there, I established a genomics program investigating the corn smut fungus and helped develop and implement the Master of Biotechnology program. Since joining the faculty at Trent University I have continued to develop my genomics research program. I established Canada wide collaborations supported by an Ontario Research Fund Research Excellence award for a project titled Genomic approaches to Mitigate Fungal Threats to Crops (GeM-FTC). This project investigates genomic interactions between fungal disease causing agents and the crop plants they infect. I have also embarked upon other fungal plant pathogen genomics projects including one funded in part by the Grain Farmers of Ontario. All projects reflect my interest in work at the intersection of basic and applied research.

Education Background:
Undergraduate degree in Botany at the University of Guelph, Masters and Ph.D. in Botany at the University of Toronto, post doctoral research at the University of Wisconsin at Madison and at the University of Toronto and then two years in industry working with Applied Biosystems, the leading company in automated DNA sequencing.

University Employment:
Joined the Department of Biology at the University of Toronto Mississauga in 1999 where I helped develop and establish the Master of Biotechnology Program with Ulli Krull and Paul Horgen.  This program is a fusion of science and business education that quickly became a model for similar programs across North America.

Joined the Forensic Science Program at Trent University in 2006 where primary teaching responsibility has been upper level courses including the development and deliver of a course in Biocrime and Bioterrorism.  In the future I will be teaching a first year Forensic Science course while continuing to work as a supervisor for 4th year student research projects.

Articles published in refereed journals

Donaldson, M.E., Meng, S., Gagarinova, A., Babu, M., Lambie, S.C., Swiadek, A., and Saville, B.J. (2013) Investigating the Ustilago maydis/Zea mays pathosystem: Transcriptional responses and novel functional aspects of a fungal calcineurin regulatory B subunit. Fungal Genetics and Biology. 58-59C: 91-104.
Donaldson, M.E., and Saville, B.J. (2013) Ustilago maydis natural antisense transcript expression alters mRNA stability and pathogenesis. Molecular Microbiology. 89(1): 29-51.

Donaldson, M.E., & Saville, B.J. (2012) Natural antisense transcripts in fungi. Molecular Microbiology. 85(3):405-417

Morrison, E.N.*, Donaldson, M.E.*, & Saville, B.J. (2012) Identification and analysis of genes expressed in the Ustilago maydis dikaryon: uncovering a novel class of pathogenesis genes. Canadian Journal of Plant Pathology/Revue Canadienne de Phytopathologie,. 34(3):417-435

Wang, X., McCallum, B.D., Fetch, T., Bakkeren, G., Marais, G.F. & Saville, B.J. (2012) Comparative microscopic and molecular analysis of Thatcher near isogenic lines with wheat leaf rust resistance genes Lr2a, Lr3, LrB or Lr9 upon challenge with different Puccinia triticina races. Plant Pathology. 62(3):698-707

Bakkeren, G., Song, X., Panwar, V., Linning, R., Wang, X., Rampitsch, C., McCallum, B.D., Fellers, J.P., & Saville, B.J. (2012) Functional genomic approaches in cereal rusts. Canadian Journal of Plant Pathology/Revue Canadienne de Phytopathologie, 34(1):3-12.

Saville, B.J., Donaldson, M.E., & Doyle, C.E. (2012) Investigating host induced meiosis in a fungal plant pathogen. In Swan (Ed.), Meiosis - Molecular Mechanisms and Cytogenetic Diversity. InTech pp 411-460

Doyle, C.E., Donaldson, M.E., Morrison, E.N., & Saville, B.J. (2011) Ustilago maydis transcript features identified through full-length cDNA analysis. Molecular Genetics and Genomics. 286(2):143-159

Xu, J., Linning, R., Fellers, J., Dickinson, M., Wenhan, Z., Antonov, I., Joly, D.L, Donaldson, M.E., Eilam, T., Anikster, Y., Banks, T., Munro, S., Mayo, M., Wynhoven, B., Ali, J., Moore, R., McCallum, B., Borodovsky, M., Saville, B.J., & Bakkeren, G. (2011) Gene discovery in EST sequences from the wheat leaf rust fungus Puccinia triticina sexual spores, asexual spores and haustoria, compared to other rust and corn smut fungi. BMC Genomics. 12(1):161

Bruce, S.A., Saville, B.J. & Emery, R.J.N. (2010) Ustilago maydis Produces Cytokinins and Abscisic Acid for Potential Regulation of Tumor Formation in Maize. Journal of Plant Growth Regulation. 30:51-63

Ho, E.C., Donaldson, M.E., & Saville, B.J. (2010) Detection of antisense RNA transcripts by strand-specific RT-PCR. In King (Ed.), Methods in Molecular Biology: RT-PCR Protocols (2nd ed.). Toronto: Humana Press.

Donaldson, M.E., & Saville, B.J. (2008) Bioinformatic identification of Ustilago maydis meiosis genes. Fungal Genetics and Biology. 45: S47-S53.

Ho, E. C., Cahill, M., & Saville, B.J. (2007) Gene discovery and transcript analysis in the corn smut pathogen Ustilago maydis: EST examination. BMC Genomics. 8:334
Kamper, J., Kahmann, R., Bolker M., Ma, L.J., Brefort, T., Saville, B.J., et al. (2006). Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis. Nature. 444:97-10

Babu, M.R., Choffe, K., & Saville B. J. (2005) Differential Gene Expression in Filamentous Cells of Ustilago maydis. Current Genetics 47(5):316-333

Zahiri, A. R., Babu, M.R. & Saville B. J. (2005) Differential gene expression during Ustilago maydis teliospore germination. Molecular Genetics and Genomics 273(5):394-403

Austin R., Provart N., Sacadura N.T., Nugent K.G., Babu M., & Saville B.J. (2004) A comparative genomic analysis of ESTs from Ustilago maydis. Functional Integrative Genomics 4:207-218

Nugent, K.G., Choffe, K., & Saville, B.J. (2004) Gene expression during Ustilago maydis diploid filamentous growth: EST library creation and analyses. Fungal Genetics and Biology. 41:349-360.

Nugent, K. & Saville, B.J. (2004) DNA-based Identification of Forensically Important Psilocybe and other Illicit Mushrooms. Forensic Science International. 140:147-157

Sacadura, N. T. and Saville, B.J. (2003) Gene Expression and EST Analysis of Ustilago maydis germinating Teliospores. Fungal Genetics and Biology. 40:47-64